Microorganisms provide valuable enzyme machinery to assemble complex molecules. Fungal prenyltransferases (PTs) typically catalyse highly regiospecific prenylation reactions that are of significant pharmaceutical interest. While the majority of PTs accepts dimethylallyl diphosphate (DMAPP), very few such enzymes can use geranyl diphosphate (GPP) or farnesyl diphosphate (FPP) as donors. This catalytic gap prohibits the wide application of PTs for structural diversification. Structure-guided molecular modelling and site-directed mutagenesis of FgaPT2 from Aspergillus fumigatus led to the identification of the gatekeeping residue Met328 responsible for the prenyl selectivity and sets the basis for creation of GPP- and FPP-accepting enzymes. Site-saturation mutagenesis of the gatekeeping residue at position 328 in FgaPT2 revealed that the size of this side chain is the determining factor for prenyl selectivity, while its hydrophobicity is crucial for allowing DMAPP and GPP to bind. Download xmod coc for pc. • For reproduction of material from NJC: Reproduced from Ref.

He is also the writer of the song 'Ripped Pants,' which he also performed instead of Tom Kenny. Trivia In the credits for ' Ripped Pants ' and ' The Chaperone. A SpongeBob SquarePants toy that ties in with this episode marketed by Fisher-Price sings and rip its pants. This episode contains the show's first nude scene. The Ripped Pants song is simalar to the intro of 'Be True To Your School' by the Beach Boys, as it is a parody of them.

XX with permission from the Centre National de la Recherche Scientifique (CNRS) and The Royal Society of Chemistry. • For reproduction of material from PCCP: Reproduced from Ref. XX with permission from the PCCP Owner Societies. • For reproduction of material from PPS: Reproduced from Ref. Download film drama korea your voice. XX with permission from the European Society for Photobiology, the European Photochemistry Association, and The Royal Society of Chemistry. • For reproduction of material from all other RSC journals and books: Reproduced from Ref. XX with permission from The Royal Society of Chemistry.

If the material has been adapted instead of reproduced from the original RSC publication 'Reproduced from' can be substituted with 'Adapted from'. In all cases the Ref. XX is the XXth reference in the list of references. If you are the author of this article you do not need to formally request permission to reproduce figures, diagrams etc.

Contained in this article in third party publications or in a thesis or dissertation provided that the correct acknowledgement is given with the reproduced material. Reproduced material should be attributed as follows.